Re: Biological inert coverslip sealing

Posted by Oshel, Philip Eugene on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Biological-inert-coverslip-sealing-tp7581614p7581618.html

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Do you have access to Canada Balsam?
Used extensively for mounting microarthropods like mites, etc. (Or it
was, anyway.

Phil

On 02/27/2014 10:52 , Antonio J Pereira wrote:

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>
> Hi all,
>
> For some purposes in live cell imaging we assemble a chamber with a coverslip on top of a slide.
> We use two halves of another coverslip to act as spacers.
>
> The  issue is the sealing. We've been using VALAP (a kind of wax) that we  melt and then use to seal all the way through the periphery of the  coverslip.
>
> It works, but it would be more convenient to have something that does not have to be heated, and that would harden just by  contact with air.
> A bit like the nail varnish used for fixed material ... but here we need something which is biologically inert.
>
> Any suggestions? Thanks a lot.
>
> Antonio
>
>
>
> António Pereira
> Chromosome Instability and Dynamics Group, Maiato lab
>
> IBMC - Institute for Molecular and Cell Biology
> Porto- Portugal
>
>
> António Pereira
> IBMC - Instituto de Biologia Molecular e Celular
> Grupo Instabilidade e Dinâmica Cromossómica
>
> Rua do Campo Alegre, 823
> 4150-180 Porto
> Portugal
>
> [phone] (ext# 1620)
> [hidden email]
>

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
[phone]