Posted by
Josef Gotzmann on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Biological-inert-coverslip-sealing-tp7581614p7581620.html
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Dear Antonio,
For this purpose we use dental cement (Picodent TwinSil 22;
http://www.picodent.de/), which is not only biologically inert, though also
can be easily removed after imaging to save the sample for post-imaging
processing.
Josef
On Thu, 27 Feb 2014 15:52:37 +0000, Antonio J Pereira <
[hidden email]>
wrote:
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>
>Hi all,
>
>For some purposes in live cell imaging we assemble a chamber with a
coverslip on top of a slide.
>We use two halves of another coverslip to act as spacers.
>
>The issue is the sealing. We've been using VALAP (a kind of wax) that we
melt and then use to seal all the way through the periphery of the coverslip.
>
>It works, but it would be more convenient to have something that does not
have to be heated, and that would harden just by contact with air.
>A bit like the nail varnish used for fixed material ... but here we need
something which is biologically inert.
>
>Any suggestions? Thanks a lot.
>
>Antonio
>
>
>
>António Pereira
>Chromosome Instability and Dynamics Group, Maiato lab
>
>IBMC - Institute for Molecular and Cell Biology
>Porto- Portugal
>
>
>António Pereira
>IBMC - Instituto de Biologia Molecular e Celular
>Grupo Instabilidade e Dinâmica Cromossómica
>
>Rua do Campo Alegre, 823
>4150-180 Porto
>Portugal
>
>+351 22 607 49 00 (ext# 1620)
>
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