Re: Biological inert coverslip sealing

Posted by Antonio Jose Pereira on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Biological-inert-coverslip-sealing-tp7581614p7581626.html

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Thank you all for the suggestions.
I have now a few things to try.



António Pereira
Chromosome Instability and Dynamics Group, Maiato lab
IBMC - Institute for Molecular and Cell Biology
Porto- Portugal


-----Confocal Microscopy List <[hidden email]> wrote: -----
To: [hidden email]
From: Christophe Leterrier
Sent by: Confocal Microscopy List
Date: 02/27/2014 08:50PM
Subject: Re: Biological inert coverslip sealing

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Hi Antonio,

We have been using dental cement on fixed samples, and as stated it should
be OK with live samples.

For live samples I prefer using EMS Diasum silicone hybridization chamber:
they exist in a lot different sizes and shapes to accommodate pretty much
any type of coverslip. Some of them have an adhesive side that sticks on
the slide, then you just need to fill the well (a few 10s-100s of µL will
do) and gently press the coverslip, then aspirate the excess medium to seal
the chamber

It is easy to remove the coverslip for further processing, and the
slide/chamber assembly is reusable. If you worry about the coverslip moving
during imaging, we have been doing single particle tracking and
super-resolution microscopy using these chambers without any noticeable
problems compared to dental cement.

Check here:
http://www.emsdiasum.com/microscopy/products/preparation/hybridization.aspx

For our 18mm round coverslips we use the one-side adhesive, 13mm wide,
0.6mm deep ref. 70-334-A:
http://www.emsdiasum.com/microscopy/products/preparation/hybridization.aspx#70334

Hope this helps,

Christophe

--
Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France




2014-02-27 16:52 GMT+01:00 Antonio J Pereira <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>
> Hi all,
>
> For some purposes in live cell imaging we assemble a chamber with a
> coverslip on top of a slide.
> We use two halves of another coverslip to act as spacers.
>
> The  issue is the sealing. We've been using VALAP (a kind of wax) that we
>  melt and then use to seal all the way through the periphery of the
>  coverslip.
>
> It works, but it would be more convenient to have something that does not
> have to be heated, and that would harden just by  contact with air.
> A bit like the nail varnish used for fixed material ... but here we need
> something which is biologically inert.
>
> Any suggestions? Thanks a lot.
>
> Antonio
>
>
>
> António Pereira
> Chromosome Instability and Dynamics Group, Maiato lab
>
> IBMC - Institute for Molecular and Cell Biology
> Porto- Portugal
>
>
> António Pereira
> IBMC - Instituto de Biologia Molecular e Celular
> Grupo Instabilidade e Dinâmica Cromossómica
>
> Rua do Campo Alegre, 823
> 4150-180 Porto
> Portugal
>
> [phone] (ext# 1620)
> [hidden email]
>