Re: imaging spheroids

Posted by Peter Gabriel Pitrone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/imaging-spheroids-tp7581903p7581905.html

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Hello Doug,

I agree with Diaspro (even though he didn't come out and directly say
it), Lightsheet microscopy would be better in this particular experiment.
I'll make a shameless attempt at self promotion as well, check out
OpenSPIM: http://www.openspim.org ;-)

Best Regards,
Pete

--
Peter Gabriel Pitrone - FRMS TechRMS
Microscopy/Imaging Specialist
Prof. Dr. Pavel Tomancak group
Max Planck Institute for
Molecular Biology and Genetics
Pfotenhauerstr. 108
01307 Dresden

http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html &
http://www.openspim.org

"If a straight line fit is required, obtain only two data points." - Anon.


On Thu, April 10, 2014 17:43, Alberto Diaspro wrote:
<|> *****
<|> To join, leave or search the confocal microscopy listserv, go to:
<|> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
<|> Post images on http://www.imgur.com and include the link in your
posting.
<|> *****
<|>
<|> We got some super resolution on spheroids
<|> http://www.nature.com/nmeth/journal/v8/n12/full/nmeth.1744.html
<|>
<|> alby
<|>
<|> www.nic.iit.it
<|>
<|>
<|> Il giorno 10 apr, 2014, alle ore 17:38, Cromey, Douglas W - (dcromey)
<|> <[hidden email]> ha scritto:
<|>
<|>> *****
<|>> To join, leave or search the confocal microscopy listserv, go to:
<|>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
<|>> Post images on http://www.imgur.com and include the link in your
<|>> posting.
<|>> *****
<|>>
<|>> I am working with a lab that is interested in doing fluorescence
<|>> microscopy on spheroid assays (clumps of cancer cells seeded and
<|>> growing inside a moderately thick collagen matrix).  They are looking
<|>> at a number of different microscopy techniques on campus.  Because our
<|>> Leica confocal was mostly configured for 2D cultured cell and tissue
<|>> sections, we have quickly discovered the working distance limitations
<|>> of our existing objectives.  Our local Leica technical representative
<|>> will be loaning us Leica's fabulous 25x/0.95 water immersion objective
<|>> (2.5mm WD) to try out.  This should help with WD issues, as well as
<|>> spherical aberration in the sample (their dishes do at least have #1.5
<|>> thickness glass coverslip bottoms).
<|>>
<|>> Has anyone else worked with these types of assays before?  Any
<|>> suggestions on the sample prep side or the imaging side to end up with
<|>> better image data?
<|>>
<|>> Thanks,
<|>> Doug
<|>>
<|>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
<|>> Douglas W. Cromey, M.S. - Associate Scientific Investigator
<|>> Dept. of Cellular & Molecular Medicine, University of Arizona
<|>> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
<|>>
<|>> office:  AHSC 4212         email: [hidden email]
<|>> voice:  520-626-2824       fax:  [phone]
<|>>
<|>> http://swehsc.pharmacy.arizona.edu/micro
<|>> Home of: "Microscopy and Imaging Resources on the WWW"
<|>>
<|>> UA Microscopy Alliance -
<|>> http://microscopy.arizona.edu<http://microscopy.arizona.edu/>
<|>