Re: imaging spheroids

Posted by Reto Fiolka on
URL: http://confocal-microscopy-list.275.s1.nabble.com/imaging-spheroids-tp7581903p7581907.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi all,
As a light sheet microscope's detection is widefield, the overall penetration depth
of the system will be limited to less than one scattering mean free path (~in the
order of 100 microns for most tissues). This might be reached in the Zeiss
youtube example and hence things get very blurry.

A two-photon scanning microscope can go up to 5 scattering path lengths, the
strehl ratio will be much reduced, but you can still see cells.

Best,
Reto