Re: imaging spheroids

Posted by Peter Gabriel Pitrone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/imaging-spheroids-tp7581903p7581908.html

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*****

Beautiful data!!

--
Peter Gabriel Pitrone - FRMS TechRMS
Microscopy/Imaging Specialist
Prof. Dr. Pavel Tomancak group
Max Planck Institute for
Molecular Biology and Genetics
Pfotenhauerstr. 108
01307 Dresden

http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html &
http://www.openspim.org

"If a straight line fit is required, obtain only two data points." - Anon.


On Thu, April 10, 2014 19:53, jens rietdorf wrote:
<|> *****
<|> To join, leave or search the confocal microscopy listserv, go to:
<|> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
<|> Post images on http://www.imgur.com and include the link in your
posting.
<|> *****
<|>
<|> FYI recent publication of lightsheet microscopy of tumor spheroids.
<|>
http://www.researchgate.net/publication/51862390_Live_cell_division_dynamics_monitoring_in_3D_large_spheroid_tumor_models_using_light_sheet_microscopy
<|> https://www.youtube.com/watch?v=72OPWhC-zy4
<|> Cheers, jens
<|>
<|>
<|> On Thu, Apr 10, 2014 at 6:40 PM, Peter Gabriel Pitrone
<|> <[hidden email]>wrote:
<|>
<|>> *****
<|>> To join, leave or search the confocal microscopy listserv, go to:
<|>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
<|>> Post images on http://www.imgur.com and include the link in your
<|>> posting.
<|>> *****
<|>>
<|>> Hello Doug,
<|>>
<|>> I agree with Diaspro (even though he didn't come out and directly say
<|>> it), Lightsheet microscopy would be better in this particular
<|>> experiment.
<|>> I'll make a shameless attempt at self promotion as well, check out
<|>> OpenSPIM: http://www.openspim.org ;-)
<|>>
<|>> Best Regards,
<|>> Pete
<|>>
<|>> --
<|>> Peter Gabriel Pitrone - FRMS TechRMS
<|>> Microscopy/Imaging Specialist
<|>> Prof. Dr. Pavel Tomancak group
<|>> Max Planck Institute for
<|>> Molecular Biology and Genetics
<|>> Pfotenhauerstr. 108
<|>> 01307 Dresden
<|>>
<|>> http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html &
<|>> http://www.openspim.org
<|>>
<|>> "If a straight line fit is required, obtain only two data points." -
<|>> Anon.
<|>>
<|>>
<|>> On Thu, April 10, 2014 17:43, Alberto Diaspro wrote:
<|>> <|> *****
<|>> <|> To join, leave or search the confocal microscopy listserv, go to:
<|>> <|> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
<|>> <|> Post images on http://www.imgur.com and include the link in your
<|>> posting.
<|>> <|> *****
<|>> <|>
<|>> <|> We got some super resolution on spheroids
<|>> <|> http://www.nature.com/nmeth/journal/v8/n12/full/nmeth.1744.html
<|>> <|>
<|>> <|> alby
<|>> <|>
<|>> <|> www.nic.iit.it
<|>> <|>
<|>> <|>
<|>> <|> Il giorno 10 apr, 2014, alle ore 17:38, Cromey, Douglas W -
<|>> (dcromey)
<|>> <|> <[hidden email]> ha scritto:
<|>> <|>
<|>> <|>> *****
<|>> <|>> To join, leave or search the confocal microscopy listserv, go to:
<|>> <|>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
<|>> <|>> Post images on http://www.imgur.com and include the link in your
<|>> <|>> posting.
<|>> <|>> *****
<|>> <|>>
<|>> <|>> I am working with a lab that is interested in doing fluorescence
<|>> <|>> microscopy on spheroid assays (clumps of cancer cells seeded and
<|>> <|>> growing inside a moderately thick collagen matrix).  They are
<|>> looking
<|>> <|>> at a number of different microscopy techniques on campus.  Because
<|>> our
<|>> <|>> Leica confocal was mostly configured for 2D cultured cell and
<|>> tissue
<|>> <|>> sections, we have quickly discovered the working distance
<|>> limitations
<|>> <|>> of our existing objectives.  Our local Leica technical
<|>> representative
<|>> <|>> will be loaning us Leica's fabulous 25x/0.95 water immersion
<|>> objective
<|>> <|>> (2.5mm WD) to try out.  This should help with WD issues, as well
<|>> as
<|>> <|>> spherical aberration in the sample (their dishes do at least have
<|>> #1.5
<|>> <|>> thickness glass coverslip bottoms).
<|>> <|>>
<|>> <|>> Has anyone else worked with these types of assays before?  Any
<|>> <|>> suggestions on the sample prep side or the imaging side to end up
<|>> with
<|>> <|>> better image data?
<|>> <|>>
<|>> <|>> Thanks,
<|>> <|>> Doug
<|>> <|>>
<|>> <|>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
<|>> <|>> Douglas W. Cromey, M.S. - Associate Scientific Investigator
<|>> <|>> Dept. of Cellular & Molecular Medicine, University of Arizona
<|>> <|>> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
<|>> <|>>
<|>> <|>> office:  AHSC 4212         email: [hidden email]
<|>> <|>> voice:  520-626-2824       fax:  [phone]
<|>> <|>>
<|>> <|>> http://swehsc.pharmacy.arizona.edu/micro
<|>> <|>> Home of: "Microscopy and Imaging Resources on the WWW"
<|>> <|>>
<|>> <|>> UA Microscopy Alliance -
<|>> <|>> http://microscopy.arizona.edu<http://microscopy.arizona.edu/>
<|>> <|>
<|>>
<|>