Re: PSF measurement using Au beads

Posted by Doube, Michael on
URL: http://confocal-microscopy-list.275.s1.nabble.com/PSF-measurement-using-Au-beads-tp7581962p7581967.html

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Hi Lu,

We've done something like this in the past, but with 80nm (IIRC) Au particles embedded in agarose and imaged with a water immersion objective (upright Zeiss LSM 780 NLO, testing alignment of all laser lines). I don't recall that the PSF was as exotic as yours - it looked like an ordinary 'Airy disk' fluorescence PSF.

Michael

<http://www.rvc.ac.uk>

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