Re: PSF measurement using Au beads

Posted by Lu Yan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/PSF-measurement-using-Au-beads-tp7581962p7581979.html

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Hi Michael,

We use 150 nm (Cytodiagnostics). We saw some nice Airy disk PSF long before
with another setup. We just diluted it at 1:1000 with ethanol, shake it
well, and then 1:1000 again with ethanol. Actually I got another question
for sample preparation, is there a sort of standard gold beads sample
preparation procedure that one can follow? My beads tend to form smaller
clusters/islands all over the cover glass (not necessarily dense) after the
ethanol evaporated off. Do I need to spin coat them?

Thanks,
Lu


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Lu Yan
Nanostructured Fibers and Nonlinear Optics Laboratory
Electrical and Computer Engineering
Boston University
8 St. Mary St., Boston, MA, 02215
[phone]
[hidden email]
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On Fri, May 2, 2014 at 3:40 AM, Doube, Michael <[hidden email]> wrote:

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>
> Hi Lu,
>
> We've done something like this in the past, but with 80nm (IIRC) Au
> particles embedded in agarose and imaged with a water immersion objective
> (upright Zeiss LSM 780 NLO, testing alignment of all laser lines). I don't
> recall that the PSF was as exotic as yours - it looked like an ordinary
> 'Airy disk' fluorescence PSF.
>
> Michael
>
> <http://www.rvc.ac.uk>
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