Re: IMAGING OF CELLULOSE

Posted by Smith, Benjamin E. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/IMAGING-OF-CELLULOSE-tp7582017p7582021.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Heyo,
    On thing you could try would be to soak the paper in fluorescein, wash out the excess, and then mount the paper in water or glycerol (which is closer to the actual refractive index of cellulose - 1.469).  Multi-photon will allow you look even further into the sample, if available.

    As others suggested, a normally more expensive option, but one that would allow you to see through the full depth of the sample with equal resolution, would be to use a microCT.

Cheers,
   Ben Smith

Benjamin E. Smith, Ph.D.
Samuel Roberts Noble Microscopy Laboratory
Research Scientist II
University of Oklahoma
Norman, OK 73019
E-mail: [hidden email]
Voice   405-325-4391
FAX  [phone]
http://www.microscopy.ou.edu/
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Dar Do [[hidden email]]
Sent: Tuesday, May 06, 2014 8:58 AM
To: [hidden email]
Subject: IMAGING OF CELLULOSE

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello,
Does anybody know of microscopy techniques for 3D imaging of paper or other
cellulose based fabrics? We have been trying with confocal microscopy and
did imaging of the surface of the outer layer of cellulose fibers, but were
unable to go through the fibers and image the underlying layers.
Best regards,
Dario
--
Dario Donnarumma
PhD student
personal blog <http://dariodonnarumma.blogspot.it/>
tel: [phone]
fax: [phone]
*Chemical Engineering @ the Micro-Scale (ChemEMS)*
*Dipartimento di Ingegneria Chimica, *
*dei Materiali e della Produzione Industriale (DICMAPI)*
*Università degli Studi di Napoli Federico II*
*Piazzale V. Tecchio 80 Napoli*
*I-80125 ITALIA*