Re: IMAGING OF CELLULOSE

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/IMAGING-OF-CELLULOSE-tp7582017p7582022.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Second harmonic microscopy does an excellent job for this sort of thing if
you happen to have access to a 2-photon microscope:
http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-28-22-2207

Craig Brideau


On Tue, May 6, 2014 at 9:50 AM, Smith, Benjamin E. <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Heyo,
>     On thing you could try would be to soak the paper in fluorescein, wash
> out the excess, and then mount the paper in water or glycerol (which is
> closer to the actual refractive index of cellulose - 1.469).  Multi-photon
> will allow you look even further into the sample, if available.
>
>     As others suggested, a normally more expensive option, but one that
> would allow you to see through the full depth of the sample with equal
> resolution, would be to use a microCT.
>
> Cheers,
>    Ben Smith
>
> Benjamin E. Smith, Ph.D.
> Samuel Roberts Noble Microscopy Laboratory
> Research Scientist II
> University of Oklahoma
> Norman, OK 73019
> E-mail: [hidden email]
> Voice   405-325-4391
> FAX  [phone]
> http://www.microscopy.ou.edu/
> ________________________________________
> From: Confocal Microscopy List [[hidden email]] on
> behalf of Dar Do [[hidden email]]
> Sent: Tuesday, May 06, 2014 8:58 AM
> To: [hidden email]
> Subject: IMAGING OF CELLULOSE
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
> Does anybody know of microscopy techniques for 3D imaging of paper or other
> cellulose based fabrics? We have been trying with confocal microscopy and
> did imaging of the surface of the outer layer of cellulose fibers, but were
> unable to go through the fibers and image the underlying layers.
> Best regards,
> Dario
> --
> Dario Donnarumma
> PhD student
> personal blog <http://dariodonnarumma.blogspot.it/>
> tel: [phone]
> fax: [phone]
> *Chemical Engineering @ the Micro-Scale (ChemEMS)*
> *Dipartimento di Ingegneria Chimica, *
> *dei Materiali e della Produzione Industriale (DICMAPI)*
> *Università degli Studi di Napoli Federico II*
> *Piazzale V. Tecchio 80 Napoli*
> *I-80125 ITALIA*
>