458 for GFP-RFP?
Posted by
mmodel on
May 06, 2014; 5:05pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/458-for-GFP-RFP-tp7582024.html
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Dear Colleagues,
We need to buy optics to do mGFP-turbo RFP FRET, and it seems that excitation at 458 might give much less direct excitation of RFP with only little sacrifice in GFP excitation. But there might be some other consideration of which I am unaware. Has anyone had any good/bad experience using 458 line for GFP? Thanks in advance!
Mike Model