Posted by
jerie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/458-for-GFP-RFP-tp7582024p7582025.html
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Dear Mike,
because the 458 line was a weak line of the Argon laser with less than 5%
of the total output power, for GFP excitation there were often limitations
to the excitation power, in particular when using low NA lenses and as a
consequence, also filter manufacturers offered less options including 458
in multiline dichroics.
So if you can provide a powerful enough laser -also to compensate for the
smaller excitation cross section- and suitable excitation optics, 458
should be well suited for GFPturbo excitation.
Greetings, Jens
On Tue, May 6, 2014 at 7:05 PM, MODEL, MICHAEL <
[hidden email]> wrote:
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>
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> Dear Colleagues,
>
> We need to buy optics to do mGFP-turbo RFP FRET, and it seems that
> excitation at 458 might give much less direct excitation of RFP with only
> little sacrifice in GFP excitation. But there might be some other
> consideration of which I am unaware. Has anyone had any good/bad experience
> using 458 line for GFP? Thanks in advance!
>
> Mike Model
>