http://confocal-microscopy-list.275.s1.nabble.com/IMAGING-OF-CELLULOSE-tp7582017p7582027.html
Yes I think second harmonic generation is useful in imaging cellulose in paper. In fact I had some experience in imaging paper ( cigarette paper) using two-photon fluorescence, second harmonic generation and coherent anti-stokes Raman. Based on the material you may be able to see strong 2-photon signal and second-harmonic signal.
Second harmonic microscopy does an excellent job for this sort of thing if you happen to have access to a 2-photon microscope:
On Tue, May 6, 2014 at 9:50 AM, Smith, Benjamin E. <
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> Heyo,
> On thing you could try would be to soak the paper in fluorescein,
> wash out the excess, and then mount the paper in water or glycerol
> (which is closer to the actual refractive index of cellulose - 1.469).
> Multi-photon will allow you look even further into the sample, if available.
>
> As others suggested, a normally more expensive option, but one
> that would allow you to see through the full depth of the sample with
> equal resolution, would be to use a microCT.
>
> Cheers,
> Ben Smith
>
> Benjamin E. Smith, Ph.D.
> Samuel Roberts Noble Microscopy Laboratory Research Scientist II
> University of Oklahoma Norman, OK 73019
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>
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> Hello,
> Does anybody know of microscopy techniques for 3D imaging of paper or
> other cellulose based fabrics? We have been trying with confocal
> microscopy and did imaging of the surface of the outer layer of
> cellulose fibers, but were unable to go through the fibers and image the underlying layers.
> Best regards,
> Dario
> --
> Dario Donnarumma
> PhD student
> personal blog <
http://dariodonnarumma.blogspot.it/>
> tel: +39 081 7682261
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