Re: IMAGING OF CELLULOSE

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/IMAGING-OF-CELLULOSE-tp7582017p7582029.html

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I actually use paper as a crude test target for 2-p systems sometimes. The
white bleach in the paper fluoresces nicely, and I can create specific
emission wavelengths by tuning the paper to make sure that each channel is
detecting properly, at least for blue and green wavelengths.

Craig



On Tue, May 6, 2014 at 1:27 PM, Ko, Alex <[hidden email]> wrote:

> Yes I think second harmonic generation is useful in imaging cellulose in
> paper. In fact I had some experience in imaging paper ( cigarette paper)
> using two-photon fluorescence, second harmonic generation and coherent
> anti-stokes Raman.  Based on the material you may be able to see strong
> 2-photon signal and second-harmonic signal.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: May-06-14 11:16 AM
> To: [hidden email]
> Subject: Re: IMAGING OF CELLULOSE
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Second harmonic microscopy does an excellent job for this sort of thing if
> you happen to have access to a 2-photon microscope:
> http://www.opticsinfobase.org/ol/abstract.cfm?uri=ol-28-22-2207
>
> Craig Brideau
>
>
> On Tue, May 6, 2014 at 9:50 AM, Smith, Benjamin E. <[hidden email]
> >wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Heyo,
> >     On thing you could try would be to soak the paper in fluorescein,
> > wash out the excess, and then mount the paper in water or glycerol
> > (which is closer to the actual refractive index of cellulose - 1.469).
> > Multi-photon will allow you look even further into the sample, if
> available.
> >
> >     As others suggested, a normally more expensive option, but one
> > that would allow you to see through the full depth of the sample with
> > equal resolution, would be to use a microCT.
> >
> > Cheers,
> >    Ben Smith
> >
> > Benjamin E. Smith, Ph.D.
> > Samuel Roberts Noble Microscopy Laboratory Research Scientist II
> > University of Oklahoma Norman, OK 73019
> > E-mail: [hidden email]
> > Voice   405-325-4391
> > FAX  [phone]
> > http://www.microscopy.ou.edu/
> > ________________________________________
> > From: Confocal Microscopy List [[hidden email]] on
> > behalf of Dar Do [[hidden email]]
> > Sent: Tuesday, May 06, 2014 8:58 AM
> > To: [hidden email]
> > Subject: IMAGING OF CELLULOSE
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hello,
> > Does anybody know of microscopy techniques for 3D imaging of paper or
> > other cellulose based fabrics? We have been trying with confocal
> > microscopy and did imaging of the surface of the outer layer of
> > cellulose fibers, but were unable to go through the fibers and image the
> underlying layers.
> > Best regards,
> > Dario
> > --
> > Dario Donnarumma
> > PhD student
> > personal blog <http://dariodonnarumma.blogspot.it/>
> > tel: [phone]
> > fax: [phone]
> > *Chemical Engineering @ the Micro-Scale (ChemEMS)* *Dipartimento di
> > Ingegneria Chimica, * *dei Materiali e della Produzione Industriale
> > (DICMAPI)* *Università degli Studi di Napoli Federico II* *Piazzale V.
> > Tecchio 80 Napoli*
> > *I-80125 ITALIA*
> >
>