Posted by
Sylvie Le Guyader on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Limiting-the-z-travel-range-on-Nikon-Ti-tp7582197p7582198.html
Hi Shalin
I assume that this happens because one user escapes the objective at the end of the imaging session and leaves the system on for the next user who then presses the reload button.
Indeed I do not understand why Nikon has chosen to lock the focus wheel when the objective has been escaped so that the only way to get in focus again is to reload.
In the meantime I would suggest you do as us: tell users to systematically shut down the software and hardware (leaving only the gas lasers on) between 2 users. This way when the next user turns the system on (it only takes a minute), the objective will be automatically escaped and the focus wheel will be unlocked.
Hope that helps :)
Med vänlig hälsning / Best regards
Sylvie
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Sylvie Le Guyader
Live Cell Imaging Unit Manager
Dept of Biosciences and Nutrition
Karolinska Institutet
Hälsovägen 7
Novum, G lift, floor 6
14157 Huddinge
Sweden
office: +46 (0) 8 5248 1107
LCI room 1: +46 (0) 8 5248 1172
LCI room 2: +46 (0) 8 5248 3542
mobile: +46 (0) 73 733 5008
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:
[hidden email]] On Behalf Of Shalin Mehta
> Sent: 17 June 2014 16:47
> To:
[hidden email]
> Subject: Limiting the z travel range on Nikon Ti
>
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>
> Hello listers,
> Our lab has damaged two objectives so far by running them into stage on our
> inverted Nikon Ti by using the 'Escape' and 'Return' for Z.
>
> Our old Nikon (finite tube length) has a mechanical stop on how close the objective
> can come to the stage and all old objectives are in great shape.
>
> Can we program the limits on Z travel on Nikon Ti? Asking our local Nikon folks
> suggests that the answer is No and we should avoid using escape/return.
>
> If this feature is not in the firmware, this is a request to Nikon Japan for such a
> feature.
>
> Thanks
> Shalin