http://confocal-microscopy-list.275.s1.nabble.com/comparison-of-lasers-for-MPM-tp7582206p7582211.html
If you are into second and third harmonic generation, it can be useful to design the system so that you can split your TiSa before you pump it to higher wavelength. This way you get one line at 900-1000 nm to excite your fluorophore and one line at 1200-1300 for THG to visualize the tissue structure.
Both SP DeepSee and Coherent TiSa/OPO can be split that way but the SP DeepSee delivers a fixed lower wavelength (1040nm which works for RFPs) and a tunable longer wavelength (690-1300nm) whereas in the Coherent system, both wavelength can be tuned (more flexible but more expensive).
If you do not need to THG, my understanding is that the SP and Coherent lasers are equivalent although I have only played with the Coherent Ultra II and the question is then if you want some compensation or not as Craig mentioned.
> -----Original Message-----
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> [mailto:
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> Sent: 18 June 2014 08:48
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> Subject: Re: comparison of lasers for MPM
>
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> Excellent point, Craig! I¹m running a core facility, so I have a lot of different users
> with different applications. So I guess from that standpoint, I¹m looking for
> thoughts on how the systems compare in range of use, ease of use, and reliability.
> Because you are right, each user will have a different application!
>
> Dr Pamela A. Young
> | Light and Optical Microscopist
> Australian Centre for Microscopy & Microanalysis
>
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> On 18/06/2014 12:55 pm, "Craig Brideau" <
[hidden email]> wrote:
>
> >*****
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http://www.imgur.com and include the link in your posting.
> >*****
> >
> >You are asking a bit of an 'apples vs. oranges' question here, in that
> >different lasers with different accessories achieve different functions.
> >Different lasers will be appropriate or inappropriate, depending on the
> >type of imaging you want to do and the types of fluorophores you want
> >to work with.
> >I always start by asking the user what non-linear imaging they want to do.
> >The usual answer is 2-photon, but some also want second harmonic
> >generation capability (SHG), and some want higher-order 3-photon
> >imaging, although this is pretty rare. This question gives clues as to
> >what pulse width and tuning range the user may require.
> >The next is what sort of tissues the user wants to image, and how deep
> >they want to go. If they want to go very deep, this indicates that
> >longer wavelength tuning ranges are appropriate, as well as dispersion
> >control with shorter pulse widths, pointing to OPO or just a
> >long-tuning Ti:Saph and pulse compression accessories. For relatively
> >shallower imaging on not particularly scattering samples, these
> >measures are not necessary.
> >Then I ask what sort of fluorophores the user is used to working with,
> >and which ones they plan to use. This will help nail down exactly what
> >excitation wavelengths will be necessary, indicating what sort of
> >tuning range will be necessary out of the laser, and whether or not an
> >OPO will be needed. For multiple fluorophores it is important to
> >determine if all of them can reasonably be excited by a single
> >wavelength, or whether a second wavelength would be needed, which again
> >points to an OPO for this situation. If the dyes the user wants will
> >all work adequately with a single wavelength than just a basic laser is
> >sufficient.
> >Finally, the experience level of the user, and whether or not the
> >system will be a 'core' system for multiple users, influences how
> >user-friendly and turnkey the system and its accessories need to be.
> >These are not the only considerations, but I hope it gives you some
> >idea of the thought processes that go towards selecting a laser.
> >
> >Craig Brideau
> >
> >
> >On Tue, Jun 17, 2014 at 8:31 PM, Pamela Young
> ><
[hidden email]>
> >wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> Post images on
http://www.imgur.com and include the link in your
> >>posting.
> >> *****
> >>
> >> Hello List,
> >>
> >> Has anyone done any comparisons of MPM lasers? Most of my experience
> >>has been with various versions of the MaiTai and the InSight DeepSee
> >>(and of course many much older lasers). So if you have thoughts on
> >>how these systems compare to the Chameleon and OPO, I would love your
> >>thoughts.
> >>
> >> Thanks,
> >> Pam
> >>
> >> Dr Pamela A. Young | Light and Optical Microscopist Australian Centre
> >> for Microscopy & Microanalysis
> >>
> >> THE UNIVERSITY OF SYDNEY
> >> Rm 116A, Madsen Building F09 | The University of Sydney | NSW | 2006
> >> | Australia T +61 2 9351 7527 | F +61 2 9351 7682 E
> >>
[hidden email]<mailto:
[hidden email]> | W
> >>
http://sydney.edu.au/acmm> >>
> >> Incorporating:
> >> Australian Microscopy & Microanalysis Research Facility (AMMRF) | W
> >>
http://www.ammrf.org.au<
http://www.ammrf.org.au/>
> >> ARC Centre of Excellence for Design in Light Metals | W
> >>
http://www.arclightmetals.org.au<
http://www.arclightmetals.org.au/>
> >>
> >> CRICOS 00026A
> >> This email plus any attachments to it are confidential. Any
> >> unauthorised use is strictly prohibited. If you receive this email in
> >> error, please delete it and any attachments.
> >>