Re: Limiting the z travel range on Nikon Ti

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Limiting-the-z-travel-range-on-Nikon-Ti-tp7582197p7582214.html

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I actually had to create some stage adapters that hold different types of
sample holders at the same height to address this issue. I have a 96 well
plate stage that puts the bottoms at the same relative location as the
standard holder puts dishes and slides. I had to 3d print the stage insert
but it works fairly well.

Craig


On Wed, Jun 18, 2014 at 9:22 AM, Glen MacDonald <[hidden email]>
wrote:

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>
> One problem with setting focus limits occurs when users bring in specimens
> mounted in special holders.  Sometimes a sample sits particularly far from
> the lens, then a collision occurs if the limit is not reset to a “normal”
> level afterwards.   We’ve even had this problem using glass bottomed
> 96-well plates where the skirt around the bottom of the plate may extend a
> large amount with some manufacturers.
>
> Glen MacDonald
>         Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center
>         Cellular Morphology Core
> Center on Human Development and Disability
> Box 357923
> University of Washington
> Seattle, WA 98195-7923  USA
> [phone]
> [hidden email]
>
>
>
>
>
>
>
> On Jun 18, 2014, at 2:44 AM, Paul Rigby <[hidden email]> wrote:
>
> > Hi All,
> > The Nikon Ti Control software also allows one to set the limits of
> travel of the X,Y stage. This is very useful to prevent users from driving
> the stage into an objective. As far as I know, the NIS software does not
> override this function.
> > Hope this helps someone.
> > Cheers
> > Paul
> >
> > Assoc. Prof. Paul Rigby
> > Centre for Microscopy, Characterisation & Analysis (M510)
> > The University of Western Australia
> > 35 Stirling Highway
> > Crawley  WA  6007
> > Australia
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> > Sent: Wednesday, 18 June 2014 2:01 AM
> > To: [hidden email]
> > Subject: Re: Limiting the z travel range on Nikon Ti
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > The Nikon TiControl software lets you communicate directly with the
> chassis. Buried in the menus is a setting for creating an upper Z-position
> limit. Once you save the setting it is written to the controller on the
> chassis. I don't know if Elements can override this or not, but that's the
> first thing I would try. (I'm still using EZC1 on my system so I don't know
> what Elements would do)
> >
> > Craig
> >
> >
> > On Tue, Jun 17, 2014 at 10:29 AM, Carol Norris <[hidden email]>
> > wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> You can also release the focus lock without moving in z by holding the
> >> ‘escape’ button in and then pressing the ‘refocus’ button. I try to
> >> impress upon my users that they should never ‘refocus’ from someone
> >> else's ‘escaped’ position.
> >>
> >> Regards,
> >> Carol
> >>
> >> Carol E. Norris, Ph.D
> >> Facility Scientist
> >> Flow Cytometry/Confocal Microscopy Facility Biotechnology/Bioservices
> >> Center University of Connecticut Unit 3149
> >> 91 N. Eagleville Rd
> >> Storrs, CT 06269-3149
> >>
> >> Phone [phone]
> >> Fax [phone]
> >>
> >> On Jun 17, 2014, at 11:46 AM, Sylvie Le Guyader
> >> <[hidden email]>
> >> wrote:
> >>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >>> Post images on http://www.imgur.com and include the link in your
> >> posting.
> >>> *****
> >>>
> >>> Another alternative for the xyz positions is to use the NIS element
> >>> user
> >> log in. This way the xyz positions are saved for each user and do not
> >> show up in the other users' account.
> >>>
> >>> I also tell users not to tick in the 'move stage to xy position' as
> >>> this
> >> easily leads to accidentally moving the stage. Instead we found that
> >> if you double click in the x or y columns, the stage moves there
> >> anyway even if the 'move stage' box is unticked. Single click simply
> >> selects the line so that it can be edited. Accidents are less likely
> >> to happen if one needs to double click.
> >>>
> >>> Med vänlig hälsning / Best regards
> >>>
> >>> Sylvie
> >>>
> >>> @@@@@@@@@@@@@@@@@@@@@@@@
> >>> Sylvie Le Guyader
> >>> Live Cell Imaging Unit Manager
> >>> Dept of Biosciences and Nutrition
> >>> Karolinska Institutet
> >>> Hälsovägen 7
> >>> Novum, G lift, floor 6
> >>> 14157 Huddinge
> >>> Sweden
> >>> office: [phone]
> >>> LCI room 1: [phone]
> >>> LCI room 2: [phone]
> >>> mobile: [phone]
> >>>
> >>>
> >>>> -----Original Message-----
> >>>> From: Confocal Microscopy List
> >>>> [mailto:[hidden email]] On Behalf Of Feinstein,
> >>>> Timothy
> >>>> Sent: 17 June 2014 17:40
> >>>> To: [hidden email]
> >>>> Subject: Re: Limiting the z travel range on Nikon Ti
> >>>>
> >>>> *****
> >>>> To join, leave or search the confocal microscopy listserv, go to:
> >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >>>> Post images on http://www.imgur.com and include the link in your
> >> posting.
> >>>> *****
> >>>>
> >>>> Hi Shalin,
> >>>>
> >>>> Using the same hardware we have also found the escape button to be
> >> quite risky
> >>>> between users.  Of equal concern is preserving saved XYZ positions.
> >>>> We have had to replace expensive bits (but not yet an objective,
> >>>> knock
> >> on
> >>>> woodŠ) when the stage automatically tries to return to a previous
> >> user¹s XYZ
> >>>> position that was set with a very different stage insert installed.
> >>>> As a result we ask all users to manually lower the objectives and
> >> delete their saved
> >>>> XYZ positions when finished.
> >>>>
> >>>> All the best,
> >>>>
> >>>>
> >>>> TF
> >>>>
> >>>> Timothy Feinstein, Ph.D. | Confocal Manager
> >>>> 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
> >>>> Phone: [phone] | Email: [hidden email]
> >>>>
> >>>>
> >>>>
> >>>>
> >>>>
> >>>>
> >>>>
> >>>> On 6/17/14, 11:20 AM, "Sylvie Le Guyader" <[hidden email]>
> >> wrote:
> >>>>
> >>>>> Hi Shalin
> >>>>>
> >>>>> I assume that this happens because one user escapes the objective
> >>>>> at the end of the imaging session and leaves the system on for the
> >>>>> next user who then presses the reload button.
> >>>>> Indeed I do not understand why Nikon has chosen to lock the focus
> >>>>> wheel when the objective has been escaped so that the only way to
> >>>>> get in focus again is to reload.
> >>>>>
> >>>>> In the meantime I would suggest you do as us: tell users to
> >>>>> systematically shut down the software and hardware (leaving only
> >>>>> the gas lasers on) between 2 users. This way when the next user
> >>>>> turns the system on (it only takes a minute), the objective will
> >>>>> be automatically escaped and the focus wheel will be unlocked.
> >>>>>
> >>>>> Hope that helps :)
> >>>>>
> >>>>> Med vänlig hälsning / Best regards
> >>>>>
> >>>>> Sylvie
> >>>>>
> >>>>> @@@@@@@@@@@@@@@@@@@@@@@@
> >>>>> Sylvie Le Guyader
> >>>>> Live Cell Imaging Unit Manager
> >>>>> Dept of Biosciences and Nutrition
> >>>>> Karolinska Institutet
> >>>>> Hälsovägen 7
> >>>>> Novum, G lift, floor 6
> >>>>> 14157 Huddinge
> >>>>> Sweden
> >>>>> office: [phone]
> >>>>> LCI room 1: [phone]
> >>>>> LCI room 2: [phone]
> >>>>> mobile: [phone]
> >>>>>
> >>>>>
> >>>>>> -----Original Message-----
> >>>>>> From: Confocal Microscopy List
> >>>>>> [mailto:[hidden email]] On Behalf Of Shalin
> >>>> Mehta
> >>>>>> Sent: 17 June 2014 16:47
> >>>>>> To: [hidden email]
> >>>>>> Subject: Limiting the z travel range on Nikon Ti
> >>>>>>
> >>>>>> *****
> >>>>>> To join, leave or search the confocal microscopy listserv, go to:
> >>>>>>
> >>>>>>
> >> http://scanmail.trustwave.com/?c=129&d=od6g0zsIvVNfDzegQ8BtCuVmNylcDld
> >>>>>> tMr
> >>>>>> Os9h-cGA&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-
> >>>> bin%2fwa%3fA0%3dconfoc
> >>>>>> alm
> >>>>>> icroscopy
> >>>>>> Post images on
> >>>>>>
> >> http://scanmail.trustwave.com/?c=129&d=od6g0zsIvVNfDzegQ8BtCuVmNylcDld
> >>>>>> tMu atpBXISA&u=http%3a%2f%2fwww%2eimgur%2ecom and include the
> >>>>>> link in your posting.
> >>>>>> *****
> >>>>>>
> >>>>>> Hello listers,
> >>>>>> Our lab has damaged two objectives so far by running them into
> >>>>>> stage on our  inverted Nikon Ti by using the 'Escape' and 'Return'
> for Z.
> >>>>>>
> >>>>>> Our old Nikon (finite tube length) has a mechanical stop on how
> >>>>>> close the objective  can come to the stage and all old objectives
> >>>>>> are in great shape.
> >>>>>>
> >>>>>> Can we program the limits on Z travel on Nikon Ti? Asking our
> >>>>>> local Nikon folks  suggests that the answer is No and we should
> >>>>>> avoid using escape/return.
> >>>>>>
> >>>>>> If this feature is not in the firmware, this is a request to
> >>>>>> Nikon Japan for such a  feature.
> >>>>>>
> >>>>>> Thanks
> >>>>>> Shalin
> >>
>