Re: Limiting the z travel range on Nikon Ti

Posted by mcammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Limiting-the-z-travel-range-on-Nikon-Ti-tp7582197p7582215.html

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We tell all users to bring the focus to the farthest position away from the sample at the end of their working session and to manually bring the focus up to the sample at the beginning of the next session.  The reason is because, as Glen points out below, people from different labs bring all sorts of different samples and assuming a normal focal position invites crashes.  This is the same on all brands of microscopes including a Nikon Ti with PFS.

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Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell:  914-309-3270   Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Glen MacDonald
Sent: Wednesday, June 18, 2014 11:22 AM
To: [hidden email]
Subject: Re: Limiting the z travel range on Nikon Ti


One problem with setting focus limits occurs when users bring in specimens mounted in special holders.  Sometimes a sample sits particularly far from the lens, then a collision occurs if the limit is not reset to a "normal" level afterwards.   We've even had this problem using glass bottomed 96-well plates where the skirt around the bottom of the plate may extend a large amount with some manufacturers.  

Glen MacDonald
        Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
        Cellular Morphology Core
Center on Human Development and Disability Box 357923 University of Washington Seattle, WA 98195-7923  USA
[phone]
[hidden email]


 

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