Re: comparison of lasers for MPM

Posted by Henthorn, Jim C. (HSC) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/comparison-of-lasers-for-MPM-tp7582206p7582217.html

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Pam,

I defiantly would not consider Coherent, since we have a Chameleon XR that died with 334 head hours and our only option is to send it to Scotland for repair for $35,000.  I was told that the service with the Mai Tai is much better.

Good luck,

Jim Henthorn
Flow and Image Cytometry Lab
975 NE 10th Street BRC 1317
Stanton L Young Biomedical Research Building
Oklahoma City, OK 73104
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http://research.ouhsc.edu/core-facilities/




On Jun 18, 2014, at 2:50 AM, Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> wrote:

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Hi Pam

If you are into second and third harmonic generation, it can be useful to design the system so that you can split your TiSa before you pump it to higher wavelength. This way you get one line at 900-1000 nm to excite your fluorophore and one line at 1200-1300 for THG to visualize the tissue structure.
Both SP DeepSee and Coherent TiSa/OPO can be split that way but the SP DeepSee delivers a fixed lower wavelength (1040nm which works for RFPs) and a tunable longer wavelength (690-1300nm) whereas in the Coherent system, both wavelength can be tuned (more flexible but more expensive).
The question is then very much if you want to image second and third harmonics.

If you do not need to THG, my understanding is that the SP and Coherent lasers are equivalent although I have only played with the Coherent Ultra II and the question is then if you want some compensation or not as Craig mentioned.

Med vänlig hälsning / Best regards

Sylvie

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-----Original Message-----
From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Pamela Young
Sent: 18 June 2014 08:48
To: [hidden email]
Subject: Re: comparison of lasers for MPM

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Excellent point, Craig!  I¹m running a core facility, so I have a lot of different users
with different applications.  So I guess from that standpoint, I¹m looking for
thoughts on how the systems compare in range of use, ease of use, and reliability.
Because you are right, each user will have a different application!

Dr Pamela A. Young
| Light and Optical Microscopist
Australian Centre for Microscopy & Microanalysis

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On 18/06/2014 12:55 pm, "Craig Brideau" <[hidden email]> wrote:

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You are asking a bit of an 'apples vs. oranges' question here, in that
different lasers with different accessories achieve different functions.
Different lasers will be appropriate or inappropriate, depending on the
type of imaging you want to do and the types of fluorophores you want
to work with.
I always start by asking the user what non-linear imaging they want to do.
The usual answer is 2-photon, but some also want second harmonic
generation capability (SHG), and some want higher-order 3-photon
imaging, although this is pretty rare. This question gives clues as to
what pulse width and tuning range the user may require.
The next is what sort of tissues the user wants to image, and how deep
they want to go. If they want to go very deep, this indicates that
longer wavelength tuning ranges are appropriate, as well as dispersion
control with shorter pulse widths, pointing to OPO or just a
long-tuning Ti:Saph and pulse compression accessories. For relatively
shallower imaging on not particularly scattering samples, these
measures are not necessary.
Then I ask what sort of fluorophores the user is used to working with,
and which ones they plan to use. This will help nail down exactly what
excitation wavelengths will be necessary, indicating what sort of
tuning range will be necessary out of the laser, and whether or not an
OPO will be needed. For multiple fluorophores it is important to
determine if all of them can reasonably be excited by a single
wavelength, or whether a second wavelength would be needed, which again
points to an OPO for this situation. If the dyes the user wants will
all work adequately with a single wavelength than just a basic laser is
sufficient.
Finally, the experience level of the user, and whether or not the
system will be a 'core' system for multiple users, influences how
user-friendly and turnkey the system and its accessories need to be.
These are not the only considerations, but I hope it gives you some
idea of the thought processes that go towards selecting a laser.

Craig Brideau


On Tue, Jun 17, 2014 at 8:31 PM, Pamela Young
<[hidden email]>
wrote:

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Hello List,

Has anyone done any comparisons of MPM lasers?  Most of my experience
has  been with various versions of the MaiTai and the InSight  DeepSee
(and of  course many much older lasers).  So if you have thoughts on
how these  systems compare to the Chameleon and OPO, I would love your
thoughts.

Thanks,
Pam

Dr Pamela A. Young | Light and Optical Microscopist Australian Centre
for Microscopy & Microanalysis

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