2P Exciting mCheery (was: Re: comparison of lasers for MPM)

Posted by ChrisWilms on
URL: http://confocal-microscopy-list.275.s1.nabble.com/comparison-of-lasers-for-MPM-tp7582206p7582226.html

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Hi Kate,

Have you tried using a wavelength around 800 nm to excite mCherry? Most red RFPs have a strong excitation peak around 800 nm and in our hands mCherry is very bright under those circumstances.

Best, Chris

> We have found that two photon excitation of mcherry is barely possible
> with these lasers and cy5 is impossible, so if your users want to have a cy5 two photon
> channel, an OPO will be necessary.