Re: Best FPs for 2-Photon

Posted by Douglas Richardson on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-FPs-for-2-Photon-tp7582250p7582255.html

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We primarily use GFP and tdTomato, they are both excited very well at
940-980.  If your green is very bright and you see some bleed through, tune
further into the IR.

-Doug


On Wed, Jun 25, 2014 at 11:07 AM, Jens-Bernhard Bosse <[hidden email]>
wrote:

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>
> Dear List,
>
> a colleague of mine wants to do 2 channel imaging on a 2-photon system
> with a max 1040 nm laser.
> What is the "best" fluorescent protein combination right now in terms of
> brightness, photostability and maturation time for this application?
>
> Thanks so much!
>
> Jens
>
> --------------------------------------------------------------
> Jens B. Bosse Ph.D.
> Enquist Lab
> Department of Molecular Biology
> and
> Princeton Neuroscience Institute
> Princeton University
> 301 Schultz Lab
> Washington Rd
> 08544 Princeton, NJ, USA
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