http://confocal-microscopy-list.275.s1.nabble.com/Best-FPs-for-2-Photon-tp7582250p7582257.html
Most of the common FP's like anywhere from 850 to 950nm. Very blue
ones tend towards 1000nm. The common ones like GFP and YFP both prefer
around 950 to 1000nm. A large swath of the Alexa dyes are also excited in
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> We primarily use GFP and tdTomato, they are both excited very well at
> 940-980. If your green is very bright and you see some bleed through, tune
> further into the IR.
>
> -Doug
>
>
> On Wed, Jun 25, 2014 at 11:07 AM, Jens-Bernhard Bosse <
[hidden email]>
> wrote:
>
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> >
> > Dear List,
> >
> > a colleague of mine wants to do 2 channel imaging on a 2-photon system
> > with a max 1040 nm laser.
> > What is the "best" fluorescent protein combination right now in terms of
> > brightness, photostability and maturation time for this application?
> >
> > Thanks so much!
> >
> > Jens
> >
> > --------------------------------------------------------------
> > Jens B. Bosse Ph.D.
> > Enquist Lab
> > Department of Molecular Biology
> > and
> > Princeton Neuroscience Institute
> > Princeton University
> > 301 Schultz Lab
> > Washington Rd
> > 08544 Princeton, NJ, USA
> >
> > Phone: +1-609-258-4990
> > Email:
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http://molbio.princeton.edu/labs/enquist/> >
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