Re: Best FPs for 2-Photon

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Best-FPs-for-2-Photon-tp7582250p7582257.html

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Most of the common FP's like anywhere from 850 to 950nm. Very blue
*emitting* fluorophores tend to prefer more towards 700nm while really red
ones tend towards 1000nm. The common ones like GFP and YFP both prefer
around 950 to 1000nm. A large swath of the Alexa dyes are also excited in
this range.

Craig


On Wed, Jun 25, 2014 at 9:33 AM, Douglas Richardson <[hidden email]
> wrote:

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> We primarily use GFP and tdTomato, they are both excited very well at
> 940-980.  If your green is very bright and you see some bleed through, tune
> further into the IR.
>
> -Doug
>
>
> On Wed, Jun 25, 2014 at 11:07 AM, Jens-Bernhard Bosse <[hidden email]>
> wrote:
>
> > *****
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> >
> > Dear List,
> >
> > a colleague of mine wants to do 2 channel imaging on a 2-photon system
> > with a max 1040 nm laser.
> > What is the "best" fluorescent protein combination right now in terms of
> > brightness, photostability and maturation time for this application?
> >
> > Thanks so much!
> >
> > Jens
> >
> > --------------------------------------------------------------
> > Jens B. Bosse Ph.D.
> > Enquist Lab
> > Department of Molecular Biology
> > and
> > Princeton Neuroscience Institute
> > Princeton University
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> > Washington Rd
> > 08544 Princeton, NJ, USA
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