Re: Zeiss 40X N.A. 1.4 Plan APo as replacement for 63X?

Posted by Ryan Schreiner on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-40X-N-A-1-4-Plan-APo-as-replacement-for-63X-tp7582324p7582326.html

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Michael,

The 40X/1.4 is a good objective for dim samples on a widefield system, I
have used it quite a lot.

The 40X/1.4 has:
24.0 brightness
161nm sampling on a 6.5µm pixel (ie Flash 4.0 CMOS)
333x333µm field of view on the Flash4.0

The 63X/1.4 has:
9.7 brightness
102nm sampling on a 6.5µm pixel (ie Flash 4.0 CMOS)
211x211µm field of view on the Flash4.0

If you are going to deconvolve the image you will find you are
undersampling quite a bit with the 40X.  When you look through the eyepiece
you will really appreciate the 2.5X improvement in brightness.

Another note is that the 1.4NA 40X and 63X have ~80% transmission from
450-750nm and the new 100X/1.4 from Zeiss has ~90%.
Also, if you are using a spinning disk you will see your axial resolution
suffer with the 40X at least with the CSU-X1 pinholes.

-Ryan Schreiner
Weill Cornell Medical College


On Fri, Jul 11, 2014 at 9:53 AM, Cammer, Michael <[hidden email]
> wrote:

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> *****
>
> Does anyone have experience with the new Zeiss 40X N.A. 1.4 PlanApo?  This
> is something I've wanted for a long time, the ability to take large fields
> of view (2k X 2k pixels) at high resolution instead of having to do tiling.
>  Also, with the new cameras that have oodles of small pixels...
>
> I'm considering replacing our 63X with this new 40X.  Any experience with
> this, other than the battle of having to explain to other scope users why
> this is not really lower magnification?
>
> Regards,
>
> Michael
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU
> Langone Medical Center
> Cell:  914-309-3270   Lab: 212-263-3208
> http://ocs.med.nyu.edu/microscopy &
> http://www.med.nyu.edu/skirball-lab/dustinlab/
>
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