Posted by
James Kerin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Claire-s-new-Mercury-Free-Microscopy-awareness-campaign-article-online-tp7582305p7582327.html
Dear Vitaly, We've not used them ourselves, but the 330nm LEDs from Q-Photonics seem (from the data sheet) to be constructed from an array of small LEDs to give a relatively large emitting area. This is OK for illuminating large samples using a condenser lens (we are aware of groups using similar devices for macro imaging), but is not ideal for episcopic illumination through the microscope objective where point intensity is more important than overall photon flux. Unfortunately we are not aware of anyone producing an indvidual high power 340nm LED with an emitting area of less than 3 mm. We provide Fura 2 LED-based illuminators by spectrally selecting the lower bandpass section of a high-power 365nm LED. This requires precise regulation of the filtered output signal, but our control electronics include this photodiode feedback and optical monitoring functionality as standard in any case. The resultant bandpass is centred at slightly longer than
340nm, but is sufficiently below the isosbestic point to give a high calcium transient and allow effective ratio calcium imaging.
http://www.cairn-research.co.uk/catalogue/illumination/led-light-sources/product/optoled-light-source-0Please let us know if you have further questions.
J. P. Kerin
Marketing Director
Cairn Research Ltd
Graveney Road
Faversham
Kent, ME13 8UP
UK
www.cairn-research.co.uk
[hidden email]
Tel: + 44 (0)1795 594507
Fax: + 44 (0)1795 594510
On 07/08/2014, 07:52pm, Vitaly Boyko (
[hidden email]) wrote:
Dear All,
Could you, please comment on your experience with diff
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Dear All,
Could you, please comment on your experience with different LED illuminators.
Is there one that covers both UV (340 nm LED for Fura-2 Ca imaging), and near-IR?
I know that Q-photonics has one at 330 nm (15 mW and 50 mW; please find the link below). Does anyone have any experience incorporating them into any LED based illumination device?
UVCLEAN: deep UV LED at 330nm
UVCLEAN: deep UV LED at 330nm
deep UV LED, deep UV sources, SETi distributor, UV light emitting diodes, UVCLEAN, LED 255nm, LED 265nm, LED 275nm, LED 280nm, LED 290nm, LE...
View on www.qphotonics.com Preview by Yahoo
Thank you,
Vitaly