Confocal Microscopy List

Re: LED illumination sources

Posted by Craig Brideau on Jul 11, 2014; 7:13pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Claire-s-new-Mercury-Free-Microscopy-awareness-campaign-article-online-tp7582305p7582330.html

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If you want to use fiber optic based deliver, also keep in mind that many
glasses used in fiber optics do not transmit wavelengths shorter than
~400nm very well.

Craig

On Fri, Jul 11, 2014 at 12:44 PM, James Kerin <[hidden email]>
wrote:

> Dear Vitaly, We've not used them ourselves, but the 330nm LEDs from
> Q-Photonics seem (from the data sheet) to be constructed from an array of
> small LEDs to give a relatively large emitting area.  This is OK for
> illuminating large samples using a condenser lens (we are aware of groups
> using similar devices for macro imaging), but is not ideal for episcopic
> illumination through the microscope objective where point intensity is more
> important than overall photon flux. Unfortunately we are not aware of
> anyone producing an indvidual high power 340nm LED with an emitting area of
> less than 3 mm. We provide Fura 2 LED-based illuminators by spectrally
> selecting the lower bandpass section of a high-power 365nm LED.
>  This requires precise regulation of the filtered output signal, but
> our control electronics include this photodiode feedback and optical
> monitoring functionality as standard in any case.  The resultant
> bandpass is centred at slightly longer than
> 340nm, but is sufficiently below the isosbestic point to give a high
> calcium transient and allow effective ratio calcium imaging.  
>
>
>
> http://www.cairn-research.co.uk/catalogue/illumination/led-light-sources/product/optoled-light-source-0
>
>
> Please let us know if you have further questions. 
>
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> J. P. Kerin
> Marketing Director
> Cairn Research Ltd
> Graveney Road
> Faversham
> Kent, ME13 8UP
> UK
>
>
> www.cairn-research.co.uk
> [hidden email]
>
> Tel: + 44 (0)1795 594507
> Fax: + 44 (0)1795 594510
>
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>  
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> On 07/08/2014, 07:52pm, Vitaly Boyko ([hidden email]) wrote:
> Dear All,
>
>
>
> Could you, please comment on your experience with diff
>
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>
>
>
> Dear All,
>
>
>
> Could you, please comment on your experience with different LED
> illuminators.
>
> Is there one that covers both UV (340 nm LED for Fura-2 Ca imaging), and
> near-IR?
>
> I know that Q-photonics has one at 330 nm (15 mW and 50 mW; please find
> the link below). Does anyone have any experience incorporating them into
> any LED based illumination device?
>
>
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> UVCLEAN: deep UV LED at 330nm
>
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>  
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>            
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> UVCLEAN: deep UV LED at 330nm
>
> deep UV LED, deep UV sources, SETi distributor, UV light emitting diodes,
> UVCLEAN, LED 255nm, LED 265nm, LED 275nm, LED 280nm, LED 290nm, LE...
>
> View on www.qphotonics.com Preview by Yahoo
>
>  
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> Thank you,
>
>
>
> Vitaly
>