Re: Zeiss 40X N.A. 1.4 Plan APo as replacement for 63X?

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-40X-N-A-1-4-Plan-APo-as-replacement-for-63X-tp7582324p7582333.html

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Hi Michael,
why 2kx2k?
If the 40x lens has a 250x250 um field of view, this would be
undersampling, pixel size 125x125 nm. If even larger field of view,
undersampling even more.
I suggest pixel size of 50x50 or 60x60 nm, and 3D deconvolution (Z step
200 nm, maybe closer),
George

On 7/11/2014 8:53 AM, Cammer, Michael wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Does anyone have experience with the new Zeiss 40X N.A. 1.4 PlanApo?  This is something I've wanted for a long time, the ability to take large fields of view (2k X 2k pixels) at high resolution instead of having to do tiling.  Also, with the new cameras that have oodles of small pixels...
>
> I'm considering replacing our 63X with this new 40X.  Any experience with this, other than the battle of having to explain to other scope users why this is not really lower magnification?
>
> Regards,
>
> Michael
>
> ===========================================================================
> Michael Cammer, Microscopy Core&  Dustin Lab , Skirball Institute, NYU Langone Medical Center
> Cell:  914-309-3270   Lab: 212-263-3208
> http://ocs.med.nyu.edu/microscopy&  http://www.med.nyu.edu/skirball-lab/dustinlab/
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George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42