http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-40X-N-A-1-4-Plan-APo-as-replacement-for-63X-tp7582324p7582336.html
Re: “ But if you want to observe really up close organels and such I would go to 63x NA1,4” .
The beauty of the scanning confocal is that you can use zoom to go from 40x -> 63x or whatever. You do not need an objective of the same NA but different magnification to achieve this.
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> Hello,
> I would like to react to George. In my humble opinion, best lateral
> optical resolution for confocal microscope is around 130 nm, so going
> to resolution 60x60 nm is overshoot and it is just wasting your drive
> space as you are not collecting any new real information. Resolutions
> of 50x50nm and such are area of superresolution microscopy. Our
> confocal Zeiss LSM 700 we have also highest resolution of 2k x 2k.
> High quality CCDs have even less, but sCMOS can get higher than 2k x
> 2k for sure. We have objective Zeiss 40x but NA 1,3 and I can tell you
> that it is the most used objective (usually we observe stem cells)
> even tho it is not NA1,4. We have also 63x1,4 NA but ppl prefer larger
> field of view of 40x. You still get great detail and many cells in one
> image. But if you want to observe really up close organels and such I
> would go to 63x NA1,4. 40x NA 1,4 seems to me like most flexible
> objective for vast majority of observations.
> Miroslav
>
>
> 2014-07-14 1:31 GMT+02:00 George McNamara <
[hidden email]>:
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>> Hi Michael,
>> why 2kx2k?
>> If the 40x lens has a 250x250 um field of view, this would be undersampling,
>> pixel size 125x125 nm. If even larger field of view, undersampling even
>> more.
>> I suggest pixel size of 50x50 or 60x60 nm, and 3D deconvolution (Z step 200
>> nm, maybe closer),
>> George
>>
>> On 7/11/2014 8:53 AM, Cammer, Michael wrote:
>>>
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>>> Does anyone have experience with the new Zeiss 40X N.A. 1.4 PlanApo? This
>>> is something I've wanted for a long time, the ability to take large fields
>>> of view (2k X 2k pixels) at high resolution instead of having to do tiling.
>>> Also, with the new cameras that have oodles of small pixels...
>>>
>>> I'm considering replacing our 63X with this new 40X. Any experience with
>>> this, other than the battle of having to explain to other scope users why
>>> this is not really lower magnification?
>>>
>>> Regards,
>>>
>>> Michael
>>>
>>>
>>> ===========================================================================
>>> Michael Cammer, Microscopy Core& Dustin Lab , Skirball Institute, NYU
>>> Langone Medical Center
>>> Cell: 914-309-3270 Lab: 212-263-3208
>>>
http://ocs.med.nyu.edu/microscopy&>>>
http://www.med.nyu.edu/skirball-lab/dustinlab/>>>
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>>
>>
>> --
>>
>>
>>
>> George McNamara, Ph.D.
>> Single Cells Analyst
>> L.J.N. Cooper Lab
>> University of Texas M.D. Anderson Cancer Center
>> Houston, TX 77054
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http://works.bepress.com/gmcnamara/42Mark B. Cannell Ph.D. FRSNZ