http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-40X-N-A-1-4-Plan-APo-as-replacement-for-63X-tp7582324p7582337.html
NA1,4. We have only NA 1,3 for 40x. It would be great to have NA 1,4
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>
> Re: “ But if you want to observe really up close organels and such I would go to 63x NA1,4” .
>
> The beauty of the scanning confocal is that you can use zoom to go from 40x -> 63x or whatever. You do not need an objective of the same NA but different magnification to achieve this.
>
> Cheers,
> Mark
>
>
> On 14/07/2014, at 8:54 am, Miroslav Varecha <
[hidden email]> wrote:
>
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>> To join, leave or search the confocal microscopy listserv, go to:
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http://www.imgur.com and include the link in your posting.
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>>
>> Hello,
>> I would like to react to George. In my humble opinion, best lateral
>> optical resolution for confocal microscope is around 130 nm, so going
>> to resolution 60x60 nm is overshoot and it is just wasting your drive
>> space as you are not collecting any new real information. Resolutions
>> of 50x50nm and such are area of superresolution microscopy. Our
>> confocal Zeiss LSM 700 we have also highest resolution of 2k x 2k.
>> High quality CCDs have even less, but sCMOS can get higher than 2k x
>> 2k for sure. We have objective Zeiss 40x but NA 1,3 and I can tell you
>> that it is the most used objective (usually we observe stem cells)
>> even tho it is not NA1,4. We have also 63x1,4 NA but ppl prefer larger
>> field of view of 40x. You still get great detail and many cells in one
>> image. But if you want to observe really up close organels and such I
>> would go to 63x NA1,4. 40x NA 1,4 seems to me like most flexible
>> objective for vast majority of observations.
>> Miroslav
>>
>>
>> 2014-07-14 1:31 GMT+02:00 George McNamara <
[hidden email]>:
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>>> To join, leave or search the confocal microscopy listserv, go to:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>> Post images on
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>>>
>>> Hi Michael,
>>> why 2kx2k?
>>> If the 40x lens has a 250x250 um field of view, this would be undersampling,
>>> pixel size 125x125 nm. If even larger field of view, undersampling even
>>> more.
>>> I suggest pixel size of 50x50 or 60x60 nm, and 3D deconvolution (Z step 200
>>> nm, maybe closer),
>>> George
>>>
>>> On 7/11/2014 8:53 AM, Cammer, Michael wrote:
>>>>
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>>>> To join, leave or search the confocal microscopy listserv, go to:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>>>> Post images on
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>>>>
>>>> Does anyone have experience with the new Zeiss 40X N.A. 1.4 PlanApo? This
>>>> is something I've wanted for a long time, the ability to take large fields
>>>> of view (2k X 2k pixels) at high resolution instead of having to do tiling.
>>>> Also, with the new cameras that have oodles of small pixels...
>>>>
>>>> I'm considering replacing our 63X with this new 40X. Any experience with
>>>> this, other than the battle of having to explain to other scope users why
>>>> this is not really lower magnification?
>>>>
>>>> Regards,
>>>>
>>>> Michael
>>>>
>>>>
>>>> ===========================================================================
>>>> Michael Cammer, Microscopy Core& Dustin Lab , Skirball Institute, NYU
>>>> Langone Medical Center
>>>> Cell: 914-309-3270 Lab: 212-263-3208
>>>>
http://ocs.med.nyu.edu/microscopy&>>>>
http://www.med.nyu.edu/skirball-lab/dustinlab/>>>>
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>>>
>>>
>>>
>>> --
>>>
>>>
>>>
>>> George McNamara, Ph.D.
>>> Single Cells Analyst
>>> L.J.N. Cooper Lab
>>> University of Texas M.D. Anderson Cancer Center
>>> Houston, TX 77054
>>> Tattletales
http://works.bepress.com/gmcnamara/42>
> Mark B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology & Pharmacology
> Medical Sciences Building
> University of Bristol
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