Re: Zeiss 40X N.A. 1.4 Plan APo as replacement for 63X?

Posted by jerie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-40X-N-A-1-4-Plan-APo-as-replacement-for-63X-tp7582324p7582342.html

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Hi Michael,

because I did not see it mentioned in the discussion so far:
To achieve the high aperture and resolution, you have to make sure
- refractive properties of all media in the light path including immersion
and embedding allow.
- overfill the back aperture of the 40x lens which is considerably wider
than that of a 63x or 100x, so collimation must fit.
I propose to compare the system PSF with the different lenses, before you
approach your facilitiy users.

A further potential drawback: Looking down the eyepiece, samples will look
much brighter than with higher-x lenses, but using suitable sampling, you
will easily burn the sample and saturate detectors, trying to achieve the
brightness seen down the eyepiece.

Cheers Jens

http://br.linkedin.com/pub/jens-rietdorf/6/4a3/189/
Skype Jens.Rietdorf


On Fri, Jul 11, 2014 at 10:53 AM, Cammer, Michael <
[hidden email]> wrote:

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>
> Does anyone have experience with the new Zeiss 40X N.A. 1.4 PlanApo?  This
> is something I've wanted for a long time, the ability to take large fields
> of view (2k X 2k pixels) at high resolution instead of having to do tiling.
>  Also, with the new cameras that have oodles of small pixels...
>
> I'm considering replacing our 63X with this new 40X.  Any experience with
> this, other than the battle of having to explain to other scope users why
> this is not really lower magnification?
>
> Regards,
>
> Michael
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU
> Langone Medical Center
> Cell:  914-309-3270   Lab: 212-263-3208
> http://ocs.med.nyu.edu/microscopy &
> http://www.med.nyu.edu/skirball-lab/dustinlab/
>
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