Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-40X-N-A-1-4-Plan-APo-as-replacement-for-63X-tp7582324p7582353.html
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Am 14.07.2014 16:28, schrieb Feinstein, Timothy:
> You are correct that even in the best case a traditional optical
> microscope can only separate point sources > 100 nm apart.
Except of course the two point sources are in different color channels.
Then you can get the two much closer together and still measure the
distance between their intensity centers very accurately. In the
two-digit nm range, depending only on your positioning accuracy.
Provided you correct for chromatic aberration.
Cheers
Steffen
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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