flash 4.0v2 for single molecule imaging

Posted by Jeff Spector on
URL: http://confocal-microscopy-list.275.s1.nabble.com/flash-4-0v2-for-single-molecule-imaging-tp7582355.html

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Greetings,
 We recently acquired a Flash4.0v2 scmos camera and have been quite happy
with it for use on our spinning disk. We are now trying to do some single
molecule imaging and have so far been unimpressed by our results. I was just
wondering if anyone has experience using this camera for single molecule (TIRF)
imaging. We are trying to look at molecules move on a surface but we don't
need incredible hight frame rates.Any advice on combinations of objective mag,
camera binning, and frame rates that will work would be most helpful..

-Jeff