AW: flash 4.0v2 for single molecule imaging

Posted by Gerhard Holst on
URL: http://confocal-microscopy-list.275.s1.nabble.com/flash-4-0v2-for-single-molecule-imaging-tp7582355p7582357.html

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Hi Jeff,

do you have any idea of the number of photons you are looking at? Binning in sCMOS does improve the signal-to-noise a little, but it is always a digital summation (that's the same effect if you reduce resolution by summing up pixel groups afterwards in the result image), therefore I doubt that it'll bring a big improvement. Bute the "binned" image will look nicer due to increased contrast.

with best regards,

Gerhard
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Dr. Gerhard Holst
Science & Research
PCO AG
Donaupark 11
93309 Kelheim, Germany
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-----Ursprüngliche Nachricht-----
Von: Confocal Microscopy List [mailto:[hidden email]] Im Auftrag von Jeff Spector
Gesendet: Donnerstag, 17. Juli 2014 01:58
An: [hidden email]
Betreff: flash 4.0v2 for single molecule imaging

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Greetings,
 We recently acquired a Flash4.0v2 scmos camera and have been quite happy
with it for use on our spinning disk. We are now trying to do some single
molecule imaging and have so far been unimpressed by our results. I was just
wondering if anyone has experience using this camera for single molecule (TIRF)
imaging. We are trying to look at molecules move on a surface but we don't
need incredible hight frame rates.Any advice on combinations of objective mag,
camera binning, and frame rates that will work would be most helpful..

-Jeff