http://confocal-microscopy-list.275.s1.nabble.com/any-ideas-how-lens-broke-tp7582371p7582378.html
We lost two objectives last week. We set the limits on our motorized stage so that the objectives could not crash, but failed to realize that you could still make them crash in unusual circumstances. We had a user doing 96 well plates with a large volume of agarose in the wells so that they had to focus way above normal with a long working distance objective to reach the sample. That crashed two other objectives in the turret into the stage as they moved around the plate. We have to now remove all the other objectives when that user is on the microscope. Dave
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> Same happened to my TIRF 100x 1.46 lens. I do not have a clue how it happened. Zeiss charged $1,500 to fix it.
>
>
> Eric Marino
> Senior Imaging Specialist
> Program in Cellular and Molecular Medicine
> Boston Children's Hospital
> 200 Longwood Ave
> WAB Room 133D
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> Lab: 617 713-8885
> Cell: 617 913-9647
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> On Jul 17, 2014, at 4:27 PM, Cammer, Michael <
[hidden email]<mailto:
[hidden email]>> wrote:
>
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> We have a Zeiss 63X N.A. 1.4 lens that broke. The top lens element appears to have collapsed into the barrel and the top is no longer retractable.
>
> Pics at
https://www.flickr.com/photos/mcammer/14492412879/ and
https://www.flickr.com/photos/mcammer/14492418378/>
> Any idea why this would have happened and if reparable.
>
> Thank you!!
>
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
> Cell: 914-309-3270 Lab: 212-263-3208
>
http://ocs.med.nyu.edu/microscopy &
http://www.med.nyu.edu/skirball-lab/dustinlab/>
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