Posted by
Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/any-ideas-how-lens-broke-tp7582371p7582379.html
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Ouch,
that happened to one of ours on an Axiovert 200M with motorized stage. The user was imaging glass bottom chambers that required a special holder and a high focus position for the 63x Water, which was the 2nd lens over on the turret. The oil lens was diagonally aimed up at a ledge protruding down from the underside of the stage. that ledge creased the nose of the objective when driven in the x-direction, right across the top of the lens and pushed it in. A good slide for teaching is of the PSFs before and after repair. Zeiss charged about $3000 to repair, and took 6 weeks. FYI, Olympus also takes 6 weeks for repairs, but are much less costly.
I’ve rebuilt stage holders so most samples are held within safe focus limits.
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Cellular Morphology Core
Center on Human Development and Disability
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
[hidden email]
On Jul 17, 2014, at 1:26 PM, Cammer, Michael <
[hidden email]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> We have a Zeiss 63X N.A. 1.4 lens that broke. The top lens element appears to have collapsed into the barrel and the top is no longer retractable.
>
> Pics at
https://www.flickr.com/photos/mcammer/14492412879/ and
https://www.flickr.com/photos/mcammer/14492418378/>
> Any idea why this would have happened and if reparable.
>
> Thank you!!
>
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
> Cell: 914-309-3270 Lab: 212-263-3208
>
http://ocs.med.nyu.edu/microscopy &
http://www.med.nyu.edu/skirball-lab/dustinlab/>
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