Re: recommended distortion target for multiphoton?

Posted by Mark Cannell-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/recommended-distortion-target-for-multiphoton-tp7582387p7582388.html

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Hi Michael

I’ve always used beads to test the microscope. They are relatively cheap and if you blow one up it don’t matter. As you say, evaporated metal targets are too easily damaged (as Jim Pauley and I discovered when testing my microscope back in ’95!)

HTH

Mark

On 22/07/2014, at 4:30 am, Michael Giacomelli <[hidden email]> wrote:

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>
> Could someone recommend a good multiphoton distortion target?
> Preferably one that is relatively robust against damage.
>
> Currently I am using a thorlabs wire grid target with fluorophore
> behind it.  The beam is attenuated by the metal, resulting in an image
> of the grid.  However, passing through the entire 1 mm slide greatly
> reduces my resolution, and unless I am extremely careful, the grid is
> ablated by the beam.
>
> Thanks,
> Michael

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

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