Re: recommended distortion target for multiphoton?

Posted by Mark Cannell-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/recommended-distortion-target-for-multiphoton-tp7582387p7582396.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Home made approach to measure file distortion.

Use a low power phase lens to measure bead position. Then switch to your high power lens and measure positions with 2P… The difference, once the average position is calculated should give field distortion…

Cheers
On 22/07/2014, at 10:06 pm, Christophe Leterrier <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Michael,
>
> If you have a good motorized stage, maybe you can scan one or a few beads
> over the whole field of view and generate a "virtual" grid?
>
> Christophe
>
>
> 2014-07-22 19:03 GMT+02:00 Michael Giacomelli <[hidden email]>:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Hi Mark,
>>
>> I'm assuming you mean beads deposited in a grid or something similar?
>> Could you point me to where you found this?
>>
>> Thanks,
>> Mike
>>
>> On Tue, Jul 22, 2014 at 3:58 AM, Mark Cannell
>> <[hidden email]> wrote:
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> Post images on http://www.imgur.com and include the link in your
>> posting.
>>> *****
>>>
>>> Hi Michael
>>>
>>> I’ve always used beads to test the microscope. They are relatively cheap
>> and if you blow one up it don’t matter. As you say, evaporated metal
>> targets are too easily damaged (as Jim Pauley and I discovered when testing
>> my microscope back in ’95!)
>>>
>>> HTH
>>>
>>> Mark
>>>
>>> On 22/07/2014, at 4:30 am, Michael Giacomelli <[hidden email]> wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> Post images on http://www.imgur.com and include the link in your
>> posting.
>>>> *****
>>>>
>>>> Could someone recommend a good multiphoton distortion target?
>>>> Preferably one that is relatively robust against damage.
>>>>
>>>> Currently I am using a thorlabs wire grid target with fluorophore
>>>> behind it.  The beam is attenuated by the metal, resulting in an image
>>>> of the grid.  However, passing through the entire 1 mm slide greatly
>>>> reduces my resolution, and unless I am extremely careful, the grid is
>>>> ablated by the beam.
>>>>
>>>> Thanks,
>>>> Michael
>>>
>>>
>>> Mark  B. Cannell Ph.D. FRSNZ
>>> Professor of Cardiac Cell Biology
>>> School of Physiology &  Pharmacology
>>> Medical Sciences Building
>>> University of Bristol
>>> Bristol
>>> BS8 1TD UK
>>>
>>> [hidden email]
>>