Re: recommended distortion target for multiphoton?

Posted by Mark Cannell-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/recommended-distortion-target-for-multiphoton-tp7582387p7582401.html

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Thinking about this a bit more, I realised that your existing grid is perfectly suitable as you don’t need 2P excitation to measure field distortion at the wavelength you are using, Just turn the power down a lot and record a reflection image,

Cheers Mark

>>
>> Hi Mark,
>>
>> I'm assuming you mean beads deposited in a grid or something similar?
>> Could you point me to where you found this?
>>
>> Thanks,
>> Mike
>>
>> On Tue, Jul 22, 2014 at 3:58 AM, Mark Cannell
>> <[hidden email]> wrote:
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>>>
>>> Hi Michael
>>>
>>> I’ve always used beads to test the microscope. They are relatively cheap
>> and if you blow one up it don’t matter. As you say, evaporated metal
>> targets are too easily damaged (as Jim Pauley and I discovered when testing
>> my microscope back in ’95!)
>>>
>>> HTH
>>>
>>> Mark
>>>
>>> On 22/07/2014, at 4:30 am, Michael Giacomelli <[hidden email]> wrote:
>>>
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>> posting.
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>>>>
>>>> Could someone recommend a good multiphoton distortion target?
>>>> Preferably one that is relatively robust against damage.
>>>>
>>>> Currently I am using a thorlabs wire grid target with fluorophore
>>>> behind it.  The beam is attenuated by the metal, resulting in an image
>>>> of the grid.  However, passing through the entire 1 mm slide greatly
>>>> reduces my resolution, and unless I am extremely careful, the grid is
>>>> ablated by the beam.
>>>>
>>>> Thanks,
>>>> Michael
>>>
>>>
>>> Mark  B. Cannell Ph.D. FRSNZ
>>> Professor of Cardiac Cell Biology
>>> School of Physiology &  Pharmacology
>>> Medical Sciences Building
>>> University of Bristol
>>> Bristol
>>> BS8 1TD UK
>>>
>>> [hidden email]
>>

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]