http://confocal-microscopy-list.275.s1.nabble.com/recommended-distortion-target-for-multiphoton-tp7582387p7582411.html
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> Unfortunately, it is extremely difficult to record the reflection mode
> image in our microscope, as the NIR light will not pass through the
> dichroic, and even if we changed out the dichroic for a beam splitter,
> out PMTs are not sensitive to NIR light.
>
> Translating a bead though is a very interesting idea. We use a high
> precision thorlabs MLS stage. I will double check the repeatability,
> but in theory I could program it to perform an extremely high
> resolution distortion measurement. Of course, getting a single bead
> onto a coverslip (or else programming software to automatically
> identify the same bead across hundreds of frames) will be somewhat
> tricky.
>
> Its a shame that getting targets for multiphoton is so difficult.
> Ideal I suppose would be something etched in glass so that I could
> flow my own fluorophore through it. I will have to look around and
> see if I can get a lithography facility to make something like that.
>
> Thanks,
> Mike
>
> On Wed, Jul 23, 2014 at 3:19 AM, Mark Cannell
> <
[hidden email]> wrote:
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> >
> > Thinking about this a bit more, I realised that your existing grid is
> perfectly suitable as you don’t need 2P excitation to measure field
> distortion at the wavelength you are using, Just turn the power down a lot
> and record a reflection image,
> >
> > Cheers Mark
> >
> >>>
> >>> Hi Mark,
> >>>
> >>> I'm assuming you mean beads deposited in a grid or something similar?
> >>> Could you point me to where you found this?
> >>>
> >>> Thanks,
> >>> Mike
> >>>
> >>> On Tue, Jul 22, 2014 at 3:58 AM, Mark Cannell
> >>> <
[hidden email]> wrote:
> >>>> *****
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> >>> posting.
> >>>> *****
> >>>>
> >>>> Hi Michael
> >>>>
> >>>> I’ve always used beads to test the microscope. They are relatively
> cheap
> >>> and if you blow one up it don’t matter. As you say, evaporated metal
> >>> targets are too easily damaged (as Jim Pauley and I discovered when
> testing
> >>> my microscope back in ’95!)
> >>>>
> >>>> HTH
> >>>>
> >>>> Mark
> >>>>
> >>>> On 22/07/2014, at 4:30 am, Michael Giacomelli <
[hidden email]> wrote:
> >>>>
> >>>>> *****
> >>>>> To join, leave or search the confocal microscopy listserv, go to:
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> >>> posting.
> >>>>> *****
> >>>>>
> >>>>> Could someone recommend a good multiphoton distortion target?
> >>>>> Preferably one that is relatively robust against damage.
> >>>>>
> >>>>> Currently I am using a thorlabs wire grid target with fluorophore
> >>>>> behind it. The beam is attenuated by the metal, resulting in an
> image
> >>>>> of the grid. However, passing through the entire 1 mm slide greatly
> >>>>> reduces my resolution, and unless I am extremely careful, the grid is
> >>>>> ablated by the beam.
> >>>>>
> >>>>> Thanks,
> >>>>> Michael
> >>>>
> >>>>
> >>>> Mark B. Cannell Ph.D. FRSNZ
> >>>> Professor of Cardiac Cell Biology
> >>>> School of Physiology & Pharmacology
> >>>> Medical Sciences Building
> >>>> University of Bristol
> >>>> Bristol
> >>>> BS8 1TD UK
> >>>>
> >>>>
[hidden email]
> >>>
> >
> >
> >
> > Mark B. Cannell Ph.D. FRSNZ
> > Professor of Cardiac Cell Biology
> > School of Physiology & Pharmacology
> > Medical Sciences Building
> > University of Bristol
> > Bristol
> > BS8 1TD UK
> >
> >
[hidden email]
>