Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CO2-grade-for-on-scope-incubator-tp7582397p7582412.html
Hi Peter, I agree with the consensus here. I think that our CO2 costs around $3 (US$) per small bottle. You should be careful however with the pressure you deliver to the mixing unit as high pressures can damage the sensor unit. This usually happens the upon the initial installation. To avoid this you can open your flow with the regulator attached and adjust the flow rate prior to attaching it to your system. Also important is having a good filter on the line.
Best of luck,
Brian D Armstrong PhD
Associate Research Professor
Director, Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Craig Brideau
Sent: Wednesday, July 23, 2014 10:53 AM
To:
[hidden email]
Subject: Re: CO2 grade for on-scope incubator?
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I agree that special CO is unnecessary. As Kurt says, the atmospheric water
is probably higher anyway. The one thing we have done in the past is swap
out carbogen (5% CO2 balance O2) for a 5% CO2 balance air mixture, because
we found 95% O2 was harsh on a particular sample.
Craig Brideau
On Tue, Jul 22, 2014 at 6:30 PM, Loralei Dewe <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> It seems to me that if this is a problem use the culture medium that has
> phenol red so you can see the pH change occuring. In all my years, I've
> never had to order specific CO for microscopy.
>
> Loralei
> On Jul 22, 2014 4:45 PM, "Peter Werner" <
[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > At our facility, we have a spinning-disk confocal system, with an
> > incubator that surrounds the entire stage and substage area. The
> question I
> > have concerns the type of CO2 I should be using for the incubator. I was
> > told by someone from the UC Berkeley Biological Imaging Facility that I
> > should be careful about the grade of carbon dioxide that I use -
> > apparently, it should be high purity and as low as possible in H2O. The
> > reason is, even if the cell cultures themselves will be fine with a lower
> > purity of CO2, CO2 forms carbonic acid in reaction with water, and the
> > acidified water content in the chamber atmosphere can be damaging to the
> > instrument over the long term, hence, the less H2O mixed in with the CO2,
> > the better. If this is the case, that would imply that "Instrument Grade"
> > or "Bone Dry" grades would be what I need to use.
> >
> > I was wondering if anybody else knew of this recommendation and followed
> > this practice. It makes sense to me why one would want to minimize
> carbonic
> > acid formation in environments the confocal system is exposed to. On the
> > other hand, it does entail some expense, not so much in terms of the gas
> > itself, but in terms of special cylinders for high-purity CO2, which must
> > be rented, or purchased at $500+ per gas cylinder.
> >
> > Let me know,
> > Peter G. Werner
> > Instructional Assistant/Lab Technician, Microscopy, Merritt College
> > SEM/AFM Lab Technician, Ohlone College
> >
[hidden email]
> >
>
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