Re: CO2 grade for on-scope incubator?

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CO2-grade-for-on-scope-incubator-tp7582397p7582417.html

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Am 23.07.2014 02:30, schrieb Loralei Dewe:
> It seems to me that if this is a problem use the culture medium that has
> phenol red so you can see the pH change occuring.

But that is not something to be universally recommended, since phenol
red is fluorescent and can cause quite a background. I'd restrict its
use to testing purposes. Or a small volume right next to your actual sample.

Steffen


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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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