Johannes Amon posted new Zeiss white paper on airyscanning at ...

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Johannes-Amon-posted-new-Zeiss-white-paper-on-airyscanning-at-tp7582420.html

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Johannes Amon posted the URL to download a new Zeiss white paper on
airyscanning

http://www.zeiss.com/airyscan 
<https://www.linkedin.com/redirect?url=http%3A%2F%2Fwww%2Ezeiss%2Ecom%2Fairyscan&urlhash=fM6m&_t=tracking_anet>

Johannes post is at

https://www.linkedin.com/groupAnswers?viewQuestionAndAnswers=&discussionID=5898093815864004612&gid=837267

and he welcomes comments.


    Airyscanning: A Novel Approach to Confocal Imaging

Johannes Amon
<https://www.linkedin.com/profile/view?id=86600201&goback=%2Egde_837267_member_5898093815864004612>Online
Communications and Marketing

It is with great pleasure that I am able to share with you a new White
Paper by ZEISS. Airyscanning is a new detection concept that uses an
array detector to oversample each Airy disk in order to gain
sensitivity, resolution and speed. I'm curious to hear what you think of
it! Download the free White Paper here:http://www.zeiss.com/airyscan 
<https://www.linkedin.com/redirect?url=http%3A%2F%2Fwww%2Ezeiss%2Ecom%2Fairyscan&urlhash=fM6m&_t=tracking_anet>


Vladimir Zhukarev and I have already commented at the linkedin group. My
comments are:



Zeiss cites some but not all of the literature of using a (small) area
array to collect light that would usually be blocked by the pinhole. I'm
thinking that Jim Pawley had this, but could be 'off by one' (Tony
Wilson, Guy Cox, etc?).

Too bad Zeiss and the other microscope companies have pathetically slow
spatial deconvolution AND on confocal microscopes deconvolution is a
separate post-processing step the user has to undertake manually (I
managed Leica confocals since 2000 and Zeiss LSM710 since 2009 ... just
trained this week on a Leica SP8 confocal, LAS AF 3.x, haven't seen all
the features, but deconvolution looks like still a separate manual step).

Further, to get the maximum benefit from a confocal microscope, when
acquiring multiple channels, should BOTH spatially deconvolve AND
spectrally unmix. See the Hoppe et al 2008 Biphys J paper,

http://www.ncbi.nlm.nih.gov/pubmed/18339754

their 3DFSR was tested with FRET specimens but the (claimed) 10x
improved SNR should apply to any specimens.

I also note that the Zeiss (claimed) 1.7x improvement could be massively
improved by acquiring 300 images (per plane) and doing 3B microscopy or
SOFI - for 3B see

http://www.coxphysics.com/3b/

for introduction to 3B (and yes, could work in 3D). 3B is also very
slow. Parallel processing is speeding up 3B, see
http://www.optnano.com/content/2/1/7/abstract

For an entry point to SOFI see

http://www.optnano.com/content/2/1/2/abstract

As I've noted on the Confocal Listserv

http://lists.umn.edu/cgi-bin/wa?A2=ind1407&L=CONFOCALMICROSCOPY&P=5920

I am psyched what parallel processing is doing (ex. NVidia TITAN Z GPU
card, Intel Phi 7120 card, lots of Phi's, as at
https://www.tacc.utexas.edu/stampede/ ) and will get even better with
2015 Knights Landing CPU's (and NVidia, IBM, HP if its "The Machine"
meets their goals, etc).

George


www.linkedin.com/in/georgemcnamara/

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George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42