Posted by
Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-LSM-880-tp7582356p7582457.html
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Am 31.07.2014 18:54, schrieb Andrew York:
> *Digital processing means bigger data files
> it was quite a relief when we switched from MSIM to instant SIM and our
> images got ~200x smaller on disk. I assume Airyscan image data is ~32x
> bigger than standard images.
Yes, it is. Plus the processed image, multiplied with what you hope to
gain in resolution, according to Nyquist. If resolution is supposed to
be 1.7x better, with the smaller pixel size in x and y required that
would be 1.7x1.7 x 33 = 95 times more data than standard.
Or is it sufficient to record with normal pixel size and the smaller
pixel size results from the reconstruction only?
Steffen
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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