Posted by
Andrew York on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-LSM-880-tp7582356p7582461.html
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This is an interesting question. With MSIM, we recorded with "normal" size
camera pixels, and "normal" size scan steps, and resampled to smaller image
pixels during our postprocessing. Our thinking was, our camera only had to
satisfy Nyquist for the emission PSF, and the scan had to satisfy Nyquist
for the excitation PSF. I'm less sure about Z, and how important it is to
take smaller z-steps.
We didn't do an especially detailed study of this, though. It's possible
you're much better off with smaller pixels or smaller steps.
On Fri, Aug 1, 2014 at 6:09 AM, Steffen Dietzel <
[hidden email]> wrote:
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> Am 31.07.2014 18:54, schrieb Andrew York:
>
> > *Digital processing means bigger data files
> > it was quite a relief when we switched from MSIM to instant SIM and our
> > images got ~200x smaller on disk. I assume Airyscan image data is ~32x
> > bigger than standard images.
>
> Yes, it is. Plus the processed image, multiplied with what you hope to
> gain in resolution, according to Nyquist. If resolution is supposed to
> be 1.7x better, with the smaller pixel size in x and y required that
> would be 1.7x1.7 x 33 = 95 times more data than standard.
>
> Or is it sufficient to record with normal pixel size and the smaller
> pixel size results from the reconstruction only?
>
> Steffen
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
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