http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-LSM-880-tp7582356p7582464.html
You can select your own PMT GaAsP detector, just the same brand that is inside that system here:
> Date: Fri, 1 Aug 2014 10:47:10 -0400
> From:
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> Subject: Re: Zeiss LSM 880
> To:
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>
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> This is an interesting question. With MSIM, we recorded with "normal" size
> camera pixels, and "normal" size scan steps, and resampled to smaller image
> pixels during our postprocessing. Our thinking was, our camera only had to
> satisfy Nyquist for the emission PSF, and the scan had to satisfy Nyquist
> for the excitation PSF. I'm less sure about Z, and how important it is to
> take smaller z-steps.
>
> We didn't do an especially detailed study of this, though. It's possible
> you're much better off with smaller pixels or smaller steps.
>
>
> On Fri, Aug 1, 2014 at 6:09 AM, Steffen Dietzel <
[hidden email]> wrote:
>
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> >
> > Am 31.07.2014 18:54, schrieb Andrew York:
> >
> > > *Digital processing means bigger data files
> > > it was quite a relief when we switched from MSIM to instant SIM and our
> > > images got ~200x smaller on disk. I assume Airyscan image data is ~32x
> > > bigger than standard images.
> >
> > Yes, it is. Plus the processed image, multiplied with what you hope to
> > gain in resolution, according to Nyquist. If resolution is supposed to
> > be 1.7x better, with the smaller pixel size in x and y required that
> > would be 1.7x1.7 x 33 = 95 times more data than standard.
> >
> > Or is it sufficient to record with normal pixel size and the smaller
> > pixel size results from the reconstruction only?
> >
> > Steffen
> >
> >
> > --
> > ------------------------------------------------------------
> > Steffen Dietzel, PD Dr. rer. nat
> > Ludwig-Maximilians-Universität München
> > Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> > Head of light microscopy
> >
> > Mail room:
> > Marchioninistr. 15, D-81377 München
> >
> > Building location:
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> >