Posted by
Sergey Tauger on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GPU-based-deconvolution-tp7582498p7582519.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Hi Brian,
I used binaries, that's a stupid fault, I should try recompiling.
There are several aspects that seriously influence deconvolution accuracy and
PSF generation speed. I commonly acquire z-stacks with depth up to 10mkm
using NA 1.2 (WI, live cells or moviol embedding) or NA 1.4 (OI, Apathy's
medium or pure glycerin embedding). I do not remember the proof (they can
easily be verified experimentally) , but G&L model is suitable if:
1. Low-aperture (<0.5) objective is used
or
2. NA<1 and imaging depth is lower than 1-2 mkm
or
3. any NA, no immersion RI mismatch and imaging depth <3 mkm
The factors that influence PSF generation time by COSM are, don't know why
so seriously
- tube length. Nikon OTL is 200, default - Zeiss 170 - is faster
- coverglass thickness (#1.5 generate faster than #0)
Thanks for oversampling factor hint, I'll use it.
Best,
Sergey