floating section antigen processing

Posted by Feinstein, Timothy on
URL: http://confocal-microscopy-list.275.s1.nabble.com/floating-section-antigen-processing-tp7582539.html

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Hello all,

This is only semi-relevant but a friend needs a good protocol for antigen
retrieval involving floating sections of previously frozen brain.  She has
seen several published protocols but the first one she tried (Jiao et al.,
J Neuroscience Methods 93: p149) produced unsatisfactory results and she
needs to keep trial and error to a minimum if possible.  If you have any
favorite protocols or can point to a reference you find especially helpful
please let me know off-line so as not to tie up the listserv.

Many thanks,


TF

Timothy Feinstein, Ph.D. | Confocal Manager
333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
Phone: [phone] | Email: [hidden email]