Posted by
Linda Barthel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/floating-section-antigen-processing-tp7582539p7582541.html
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Tim,
What is she trying to localize? A 2N HCl pretreatment can work on some
cases. It isn't as reliable as hot sodium citrate but it could easily be
applied to sections. Brain tissue is very heavy in fatty tissues so adding
a mix of triton, tween and DMSO to the ICC reagents may help
Linda Barthel
University of Michigan
Dept. Molecular, Cellular and Devlp. Bio.
On Monday, August 18, 2014, Feinstein, Timothy <
[hidden email]>
wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> This is only semi-relevant but a friend needs a good protocol for antigen
> retrieval involving floating sections of previously frozen brain. She has
> seen several published protocols but the first one she tried (Jiao et al.,
> J Neuroscience Methods 93: p149) produced unsatisfactory results and she
> needs to keep trial and error to a minimum if possible. If you have any
> favorite protocols or can point to a reference you find especially helpful
> please let me know off-line so as not to tie up the listserv.
>
> Many thanks,
>
>
> TF
>
> Timothy Feinstein, Ph.D. | Confocal Manager
> 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
> Phone: 616-234-5819 | Email:
[hidden email]
>
--
Linda Barthel, M.S.
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