Re: floating section antigen processing

Posted by Michael Delannoy on
URL: http://confocal-microscopy-list.275.s1.nabble.com/floating-section-antigen-processing-tp7582539p7582544.html

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Timothy,
Try this reference:
Microscoscopy Immunohistochemsitry, and Antigen Retrieval Methods
For Light and Electron Microscopy

By: M.A.Hayat
2002  ISBN 0-306-46770-4
Kluwer Academic/Plenum Publishers N.Y.

Good luck,
Michael Delannoy

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Feinstein, Timothy
Sent: Monday, August 18, 2014 7:45 PM
To: [hidden email]
Subject: floating section antigen processing

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Hello all,

This is only semi-relevant but a friend needs a good protocol for antigen
retrieval involving floating sections of previously frozen brain.  She has
seen several published protocols but the first one she tried (Jiao et al., J
Neuroscience Methods 93: p149) produced unsatisfactory results and she needs
to keep trial and error to a minimum if possible.  If you have any favorite
protocols or can point to a reference you find especially helpful please let
me know off-line so as not to tie up the listserv.

Many thanks,


TF

Timothy Feinstein, Ph.D. | Confocal Manager
333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
Phone: [phone] | Email: [hidden email]