imaging zebrafish

Posted by megha kumar on
URL: http://confocal-microscopy-list.275.s1.nabble.com/imaging-zebrafish-tp7582876.html

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Hello everyone!
I am imaging the 256 and 512 cell stage of zebrafish using confocal (Leica
TCS SP5 II). The embryos are stained with alpha and gamma tubulin antibody.
I take z stacks and reconstruct the entire embryo using a 3D software. I am
unable to visualize the deeper cells below although I catch the first top
most layer pretty well, which gives me a hollow half dome shape in 3D. Any
ideas why I dont see the deeper cells at 512 cell stage? Is this a
microscope issue or penetration is not deep enough or something else?

--
Megha Kumar, Ph.D.
Young Investigator
Regional Center for Biotechnology
180 Udyog Vihar phase I
Gurgaon 122016
India

ph: 8826422770