Re: imaging zebrafish

Posted by Martin Wessendorf-2 on Nov 11, 2014; 1:45pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/imaging-zebrafish-tp7582876p7582880.html

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Dear Dr. Kumar--

What wavelength fluorophore are you using?  I would expect that you'd
have best results with far-red--there'd be less scattering.

Good luck!

Martin Wessendorf


On 11/11/2014 1:58 AM, megha kumar wrote:
> Hello everyone!
> I am imaging the 256 and 512 cell stage of zebrafish using confocal (Leica
> TCS SP5 II). The embryos are stained with alpha and gamma tubulin antibody.
> I take z stacks and reconstruct the entire embryo using a 3D software. I am
> unable to visualize the deeper cells below although I catch the first top
> most layer pretty well, which gives me a hollow half dome shape in 3D. Any
> ideas why I dont see the deeper cells at 512 cell stage? Is this a
> microscope issue or penetration is not deep enough or something else?
>

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